Effect of casein hydrolysates on the survival of protective cultures of Lactococcus lactis and Lactobacillus sakei in PVOH films

The aim of this work has been to explore the potential blending polyvinyl alcohol (PVOH) with casein hydrolysates (HCas) obtain self-standing films capable act as carriers lactic acid bacteria (LAB) biocontrol agents against food pathogens. For purpose, PVOH was blended HCas at different weight ratios and blends were incorporated Lactococcus lactis Lactobacillus sakei. Blending resulted in modification some functional properties whereas did not change them. Moreover, incorporation an increase cell viability after film casting long-term storage, also antilisterial properties. These results could be related capacity bacterial autoaggregation during drying process when added, observed by fluorescence light microscopy. Blends used active packaging foods.